Fig. 3

TREM2 silencing exacerbates stroke outcomes. Tissue homogenates from the ischemic cerebral hemisphere (ischemic core and penumbra) of mice that underwent MCAO and 7 days of the indicated treatment were subjected to quantitative real-time PCR (a) and western blotting (b) and densitometric analysis (c) of TREM2 relative to GAPDH proteins.d Immunofluorescence labeling with NeuN (red) and TUNEL (green) of the indicated groups in vivo. e The percentage of apoptotic neurons was calculated using image analysis software. Quantified data are shown as mean ± SEM; n = 6. Scale bar, 50 μm. f Representative samples of TTC-stained brain sections showing the infarcted areas in white. g The infarct volume was determined using image analysis and expressed as a percentage of the whole cerebral tissue. Data are presented as mean ± SEM; n = 5. h Neurological function assessed by the Modified Garcia Score. Data are presented as mean ± SEM; n = 16; *P < 0.05 compared with sham; ^# P < 0.05 compared with the siRNA control. MCAO 7d, 7 days after mice were subjected to MCAO only; Con siRNA, mice subjected to MCAO followed by injections of scrambled siRNA for 7 days; TREM2 siRNA, mice subjected to MCAO followed by TREM2 siRNA injections for 7 days