Fig. 4

TREM2 is critical for the inflammatory response following OGDR. a OGDR was induced in cells, and the cells were transfected 6h and 12h later with TREM2 siRNA. TREM2 and GAPDH protein levels were measured by western blotting. Densitometric analysis of TREM2 relative to GAPDH is shown as mean ± SEM; n = 5; *P < 0.05 compared with control group; ^# P < 0.05 compared with siRNA control in cells transfected 6h after OGDR (OGDR6h + Con siRNA); ^Δ P < 0.05 compared with siRNA control in cells transfected 12h after OGDR (OGDR12h + Con siRNA). b Quantitative real-time PCR was performed to detect the mRNA levels of TNF-α, IL-1β, iNOS, and IL-10 in cells transfected with TREM2 siRNA 6h and 12h after OGDR. Quantified fold changes are shown as mean ± SEM; n = 6; *P < 0.05 compared with control group; ^# P < 0.05 compared with OGDR 6h + Con siRNA group; ^Δ P < 0.05 compared with OGDR 12h + Con siRNA group. c Overexpression of TREM2 6h and 12h after OGDR. TREM2 protein levels were measured by western blotting. Densitometric analysis of TREM2 relative to GAPDH is shown as mean ± SEM; n = 5; *P < 0.05 compared with control group; ^# P < 0.05 compared with pcDNA vector control transfected 6h after OGDR (pcDNA group); ^Δ P < 0.05 compared with pcDNA vector control transfected 12h after OGDR. d Quantitative real-time PCR was performed to detect the mRNA levels of inflammatory factors following TREM2 overexpression 6h and 12h after OGDR. Quantified fold changes are shown as mean ± SEM; n = 6; *P < 0.05 compared with control group; ^# P < 0.05 compared with pcDNA vector control transfected 6h after OGDR group; ^Δ P < 0.05 compared with pcDNA vector control transfected 12h after OGDR group