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Fig. 6 | Molecular Brain

Fig. 6

From: Turtle interacts with borderless in regulating glial extension and axon ensheathment

Fig. 6

Loss of tutl in R cells disrupts axon ensheathment. a-d Third-instar larval optic stalks were analyzed by electron microscopy. False coloring indicates R-cell axonal profile. a In wild type, eight R-cell axons from the same ommatidia form a single bundle, which is wrapped by WG processes within the optic stalk. Note that the central R8 axon is surrounded by other R-cell axons, and does not contact WG processes. b and c In tutl 01085 eye-specific mosaic individuals, in which tutl was mutated in R cells but not in WG, two types of defects in axonal ensheatment were observed. First, some R-cell axonal fascicles were not separated by WG processes (b). And second, some WG processes extended aberrantly within the fascicle to contact the central R8 axon (arrows) (c). d The phenotype in b was quantified. Compared to that of wild type, loss of tutl in R cells significantly increased the number of fascicles that were not separated by glial processes (*, p < 0.05). e The phenotype in c was quantified. Loss of tutl in R cells led to ectopic extension of glia processes that contact the central R8 axon (*, p < 0.05). Two-tailed t-tests were used. For each genotype, three optic stalks were analyzed by electron microscopy. Error bars indicate SEM. Scale bar: 200 nm

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