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Fig. 1 | Molecular Brain

Fig. 1

From: Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanisms

Fig. 1

Validation of pluripotency in reprogrammed cells. a Representative image of alkaline phosphatase (AP) staining of iPSCs. b, c RT-PCR analysis of OCT4 and SOX2 expression in patient and control iPSC lines. Expression was normalized to GAPDH expression. d, e, f Immunocytochemistry analysis of OCT4 and SSEA4 in iPSCs and the corresponding Hoechst stain. g, h, i Immunocytochemistry analysis of SOX2 and TRA180 in iPSCs and the corresponding Hoechst stain. j, l & n Expression of Smooth Muscle Actin (SMA), Alpha Fetal Protein (AFP) And Beta Tubulin (TUJ1) confirmed tri-lineage differentiation potential of iPSCs with respective Hoechst stains shown in (k), (m) and (o). Scale bars 100 μm

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Molecular Brain

ISSN: 1756-6606