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Fig. 2 | Molecular Brain

Fig. 2

From: Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanisms

Fig. 2

Patient ACM decreases viability of control and patient iPSC-derived motor neurons. Astrocytes and motor neurons were generated from iPSCs. a Representative image of GFAP staining confirms the generation of astrocytes from MNPs, with corresponding DAPI stain shown in (b). c Representative image of anti-MNX1 staining confirmed the generation of motor neurons derived from iPSCs with corresponding DAPI stain shown in (d). For quantification of motor neuron survival motor neurons were cultured in the presence of ACM for 5 days, after which cells were fixed and stained for anti-MNX1 (e), DAPI (f) and TUJ1 (g). h Quantification of motor neurons derived from control and patient iPSC lines iPSC1c1 and iPS31c8. i Quantification of control iPSC1c1-derived MNX1 positive motor neurons after 5 days of culture with control (n = 3) and patient (n = 4) ACM. j Quantification of patient iPS31c8-derived MNX1 positive motor neurons after 5 days of culture with control (n = 3) and patient (n = 4) ACM. *p < 0.05 and **p < 0.01 were considered statistically significant. Error bars represent standard error of mean. Scale bars 100 μm

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