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Fig. 3 | Molecular Brain

Fig. 3

From: Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanisms

Fig. 3

ACM alters expression of autophagy related proteins in HEK293T cells. HEK293T cells were cultured in ACM from control (n = 3) and patient (n = 4) iPSC-derived astrocytes for 5, after which protein was harvested for western blot analysis. Cells were also cultured in DMEM + 10% FBS with daily media changes (Fresh medium) and DMEM + 10% FBS without changing the medium for 5 days (Normal Medium). a, d, g Western blot analyses of pULK1, BECLIN-1, ATG3, ATG12, LC3-I and LC3-II. b, c, e, f, h, i Densitometry of western blot analyses. All values were normalized to β-Actin expression. *p < 0.05was considered statistically significant. Error bars represent standard error of mean

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Molecular Brain

ISSN: 1756-6606