Fig. 4

Patient ACM increases accumulation of p62 puncta in HEK293T cells. HEK293T cells were treated with ACM from control and patient iPSC-derived astrocytes for 5 days and subsequently fixed in 4% paraformaldehyde and stained for p62 expression. p62 puncta were quantified using automated image analysis. a Representative image of p62 expression in HEK293T cells treated with control ACM (n = 3), with Hoechst staining shown in (b). c Representative image of p62 expression in HEK293T cells treated with patient ACM (n = 4), with Hoechst staining shown in (d). e Quantification of p62 puncta in cells treated with ACM. *p < 0.05 was considered statistically significant. Error bars represent standard error of mean