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Fig. 5 | Molecular Brain

Fig. 5

From: Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanisms

Fig. 5

Patient ACM increases accumulation of SOD1. HEK293T cells were cultured in ACM from control (n = 3) and patient (n = 4) iPSC-derived astrocytes for 5 days, after which protein was harvested for western blot analysis. Cells were also cultured in DMEM + 10% FBS with daily media changes (Fresh medium) and DMEM + 10% FBS without changing the medium for 5 days (Normal Medium). a Western blot analysis of SOD1 and TDP-43. b, c Densitometry analyses of SOD1 and TDP-43 expression. All values were normalized to β-Actin expression. *p < 0.05 was considered statistically significant. Error bars represent standard error of mean

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Molecular Brain

ISSN: 1756-6606