Fig. 3

Susceptibility of GABAergic neurons to ketamine-induced apoptosis is age-dependent. a, b Representative confocal images of S1 sections labelled with CC3 (red), GAD67-GFP (green) and TOPRO (blue) at P5 (a) and P9 (b). Cortical layers are indicated by Roman numerals. Conditions as indicated. CC3⁺ cells co-localizing with GAD67-GFP are indicated by white arrowheads, while those not co-localizing are indicated by white arrows; scale bar is 100 μm. Zoomed images of boxed regions are presented to the right of each panel; scale bar is 50 μm. (c-f) Proportion of CC3⁺ cells co-labelling with GAD67-GFP in all cortical layers (c), layers II – IV (d), layer V (e) and layers I and VI (f). 4–6 mice were used per condition. ^** P < 0.01, ^*** P < 0.001, using two-way ANOVA followed by Bonferroni post hoc tests. Data are shown as the mean ± SEM