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Fig. 3 | Molecular Brain

Fig. 3

From: Ketamine-induced apoptosis in the mouse cerebral cortex follows similar characteristic of physiological apoptosis and can be regulated by neuronal activity

Fig. 3

Susceptibility of GABAergic neurons to ketamine-induced apoptosis is age-dependent. a, b Representative confocal images of S1 sections labelled with CC3 (red), GAD67-GFP (green) and TOPRO (blue) at P5 (a) and P9 (b). Cortical layers are indicated by Roman numerals. Conditions as indicated. CC3+ cells co-localizing with GAD67-GFP are indicated by white arrowheads, while those not co-localizing are indicated by white arrows; scale bar is 100 μm. Zoomed images of boxed regions are presented to the right of each panel; scale bar is 50 μm. (c-f) Proportion of CC3+ cells co-labelling with GAD67-GFP in all cortical layers (c), layers II – IV (d), layer V (e) and layers I and VI (f). 4–6 mice were used per condition. ** P < 0.01, *** P < 0.001, using two-way ANOVA followed by Bonferroni post hoc tests. Data are shown as the mean ± SEM

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