Fig. 5

Elevating neuronal activity with hM3Dq/CNO attenuated ketamine-induced apoptosis. a Schematic of the experimental procedure. b Representative confocal images of S1 sections from P9 mice labelled with CC3 (red), hM3Dq-mCitrine (green), and Nissl (blue). Conditions as indicated. Scale bar is 200 μm. c Quantitation of CC3⁺ cell number per mm² S1, treatment conditions as indicated. hM3Dq/CNO treatment significantly reduced the number of CC3⁺ cells in the Keta + Dex group, but not in the PBS group. ^** P < 0.01, ^*** P < 0.001, n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc test. d Proportion of hM3Dq-mCitrine⁺ cells that are CC3⁺, conditions as indicated. ^*** P < 0.001, n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc test. e Distribution of CC3⁺ cells in different cortical layers. n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc test. 4 mice were used per condition, 10 brain slices from each mouse were quantitated. Data are shown as the mean ± SEM