Fig. 7

EE rearing altered the proportion of apoptotic GABAergic neurons. a Representative confocal images of S1 sections labeled with CC3 (red), GAD67-GFP (green) and TOPRO (blue). CC3⁺ cells co-localizing with GAD67-GFP are indicated by white arrowheads, while those not co-localizing are indicated by white arrows. Conditions as indicated; scale bar is 100 μm. Zoomed image of boxed regions are shown to the right of each panel; scale bar is 50 μm. b-e Proportion of CC3⁺ cells co-labelling with GAD67-GFP in all cortical layers (b), layers II – IV (c), layer V (d) and layers I and VI (e). 3–5 mice were used per condition. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, n.s., not significant, using two-way ANOVA followed by Bonferroni post hoc tests. Data are shown as the mean ± SEM