Fig. 1

Bath application of 5-HT reduced the amplitude of evoked EPSC. a Schematic diagram of a slice illustrating the placement of a whole-cell patch recording and stimulating electrode in anterior cingulate cortex. b Current-clamp recordings to identify pyramidal neurons (left) and interneurons (right) by step current injection. c An example showing the time course of recorded pyramidal neurons in layer II/III of ACC after application of 5-HT (5 μM) and washout. The insets showed averaged trace of six eEPSCs about baseline, application of 5-HT and washout. d The averaged time course of recorded neurons (n = 9 neurons/6 mice) in ACC. Bath application of 5-HT (5 μM) led to a gradual reduction of eEPSC amplitude and washing out 5-HT with ACSF caused the rise of eEPSC amplitude again. e Summary of the effect of 5-HT (5 μM) and washout on the amplitude of eEPSC. Bath application of 5-HT significantly reduced eEPSCs amplitude and washing the system with fresh ACSF partially but evidently reversed the decreased eEPSC (n = 9 neurons/6 mice). ^** p < 0.01 compared to baseline; ^# p < 0.05 compared to the group of 5-HT