Fig. 5

5-HT- mediated reduction of eEPSC amplitude was inhibited by GDP-β-S. a The averaged time course showing the change of eEPSC amplitude after bath application of 5-HT (5 μM) in the recorded ACC neurons with or without the internal solution containing GDP-β-S (1 mM). The red dots indicated the recording with GDP-β-S. The grey dots indicated the recording without GDP-β-S (data from Figure 1). b Summary result of effect of 5-HT on eEPSC in the presence of GDP-β-S (n = 9 neurons/5 mice) or absence of GDP-β-S in the recording internal solution (n = 9 neurons/6 mice). ^* p < 0.05 compared to baseline; ^# p < 0.05 compared to the phase of 5-HT application with GDP-β-S in the internal solution of recording electrodes