Fig. 6
5-HT1A receptor was involved in the inhibiting effect of 5-HT on synaptic transmission. a One sample neuron showing the time course of the change of eEPSC amplitude after 5-HT (5 μM) application in the presence of 5-HT1A receptor antagonist, NAN-190 (5 μM). The insets showed averaged trace of six eEPSCs. b The averaged time course of recorded neurons (n = 9 neurons/6 mice) in ACC after application of 5-HT in the presence or absence of NAN-190. The red dots indicated the recording in the presence of NAN-190. The grey dots indicated the recording in the absence of NAN-190 (control, data from Figure 1). c One sample neuron illustrating the time course of the change of PPR after 5-HT application in the presence of NAN-190. The insets showed averaged trace of six sweeps. d Summary plot showing the effect of 5-HT (5 μM) on eEPSC amplitude (left) and PPR (right) when NAN-190 (5 μM) was added into the system beforehand. NAN-190 partially inhibited the reduction of eEPSC caused by 5-HT, and there was no big difference between the PPR before and after the application of 5-HT in the presence of NAN-190. n = 9 neurons/6 mice; * p < 0.05 compared to baseline. # p < 0.05 compared to the phase of 5-HT application in the recording with NAN-190 prior adding to the system