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Fig. 1 | Molecular Brain

Fig. 1

From: Mechano growth factor, a splice variant of IGF-1, promotes neurogenesis in the aging mouse brain

Fig. 1

a Scheme for generating the inducible overexpression of MGF based on the Lac operon system. Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecular mimic of allolactose, a lactosemetabolite that triggers transcription of the Lac operon. We generated a new inducible transgene utilizing the promoter from the Huntington’s disease (HD) locus that was modified to include 2 Lac operators flanking the transcription start site to drive expression of the MGF-EGFP-Luc chimeric cDNA. When the mouse is fed with IPTG dissolved in daily water, the Lac operon is de-repressed and MGF is transcribed. The HDOS promoter drives expression specifically in neurons. GFP expression, which represents the MGF transgene, was restricted to neurons of selected area including (b) spinal cord and (c) hippocampus (negative controls not shown)

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