Fig. 3
From: Mechano growth factor, a splice variant of IGF-1, promotes neurogenesis in the aging mouse brain
Characterization of endogenous MGF-expressing cells in the brain. a In the SVZ, MGF positive cells also express GFAP, which is a marker of neural stem cells in the SVZ. b Immature neurons in the SVZ were positive for both DCX and (c) PSA-NCAM expression and co-expressed MGF. These double positive neurons were in the RMS. d In the hippocampus, astrocyte-like MGF positive neurons did not express Nestin in the radial-glial like neural progenitors (type-1 NPCs) in DG area. e Nestin-positive amplifying neural progenitors in DG were positive for MGF in SGZ. f MGF was also expressed in the cytoplasm of β-Tubulin III positive neurons in the neurogenic region of DG. β-Tubulin III is one of the earliest markers seen in immature neurons. g BrdU-positive proliferating cells are also co-localizing with MGF in SVZ of LV in the brain. [Red is MGF, Green is GFAP (in a), DCX (in b), PSA-NCAM (in c), Nestin (in e), β-Tubulin III (in f) and BrdU (in g)]. (Scale bar (in a-g) 100 um)