Fig. 1
Hemin administration induces ICH injury. WT mice were administered 250 nmol of hemin in the striatum. a At 24 h after hemin injection, each brain was sectioned and used for cresyl violet staining to measure brain damage. Representative images are shown. Scale bar: 1 mm. b The injury volume (mm3) was calculated by multiplying the section thickness by the injured hemorrhagic area (** p < 0.01 vs. control mice, n = 5). c At 24 h following hemin injection, neurological deficits in the injured mice were evaluated using a 28-point neurological scoring system (** p < 0.01 vs. control mice). d-e BBB permeability after hemin injection was tested using Evans blue staining (D) from which the dye-stained volume was calculated (E) (** p < 0.01 vs. control mice, n = 4). f Total RNA was prepared from the injured brain hemispheres at 6 h after hemin injection (n = 3) and used for quantitative real-time RT-PCR to measure TLR2 mRNA levels (* p < 0.05 vs. control mice). For all graphs, the data are expressed as mean ± SEM