Fig. 2

The level of P-MAPK activity of Nf1 ^+/− mice in hippocampus shows different circadian oscillations compared with WT mice. a pErk1/2 expression in Nf1 ^+/− mice and littermates WT mice were evaluated by western blot analysis at day and night. pErk1/2, normalized to Erk, the statistical quantification is shown in the right panel (Nf1 ^+/− mice: n = 6 mice per time point, WT mice: n = 3 mice per time point, all groups normalized to WT in day), *p < 0.05, **p < 0.01. b pErk1/2 expression in C57BL/6 mice were evaluated by western blot analysis at day and night (10:30–11:00 a.m. and 10:30–11:00 p.m.). pErk1/2, normalized to Erk, the statistical quantification is shown in the right panel (n = 10 mice per time point, all groups normalized to C57BL/6in day), **p < 0.01. c pErk1/2 protein expression in 129 T2/SvEmsJ mice. pErk1/2 expression level, normalized to total Erk protein in the hippocampus (n = 7 mice per time point, all groups normalized to129T2/SvEmsJ in day). d pErk1/2 expression in mice with hybrid background of 129 T2/SvEmsJ-C57BL/6 mice were evaluated by western blot analysis at day and night. pErk1/2 expression level, normalized to total Erk protein in the hippocampus (n = 3 mice per time point, all groups normalized to mice in day)