Skip to main content
Fig. 2 | Molecular Brain

Fig. 2

From: O-GlcNAc regulation of autophagy and α-synuclein homeostasis; implications for Parkinson’s disease

Fig. 2

Inhibition of OGA by thiamet G (TG) decreases autophagic flux. (a&b) Western blot analysis of protein extracts obtained from rat cortical neurons following exposure to thiamet G (0.25 μM) for 24 h (a) and 7 d (b) in the presence or absence of chloroquine (CQ). For detection of LC3, 5 μg protein was separated on a 12% SDS-PAGE and subjected to immunoblotting with anti-LC3 antibody. β-actin was used as a loading control. (c-d) Bar graphs represent the quantification of the band intensities for LC3II using image J software 1.48e NIH, USA. (e-f) The calculated values of LC3-II with CQ minus LC3-II without CQ (Autophagic Flux) from a-b and normalized to 0 μM TG. (g-h) LC3 puncta were detected by immunocytochemistry and quantified as puncta/cell. Scale bar = 10 μm. For all panels, Data = mean ± SEM (n = 3). *p < 0.05 compared to 0 μM thiamet G without CQ. For Panel C-D, #p < 0.05 compared to without CQ. Results were analyzed by ANOVA followed by Bonferroni’s Multiple Comparison post-hoc test

Back to article page