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Fig. 3 | Molecular Brain

Fig. 3

From: O-GlcNAc regulation of autophagy and α-synuclein homeostasis; implications for Parkinson’s disease

Fig. 3

Protein O-GlcNAcylation in Parkinson’s disease postmortem brains. (a) Western blot analysis of protein extracts obtained from postmortem temporal cortical samples obtained from non-disease (ND) and stage II-IV Parkinson’s disease (PDII, PDIII and PDIV) specimens. 10 μg protein was separated on a 7% SDS-PAGE, and subjected to immunoblotting using CTD110.6 monoclonal antibody against O-GlcNAcylated proteins. β-actin antibody was used as a loading control. (b) Quantification of the band intensities for O-GlcNAc modified proteins from (a) using image J software 1.48e NIH, USA. n = 6–12 samples. There is a significant difference in median (p = 0.0095), with PDIV, PDIII, and PDII compared to ND (p = 0.0086, 0.0120, 0.0351, respectively, Wilcoxon). The p value corrected for multiple tests are: p = 0.0258, 0.0360, and 0.1053, respectively. There were no significant differences between different stages of PD by ANOVA (PDII-IV stage). * p = 0.0095

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