Fig. 4
From: O-GlcNAc regulation of autophagy and α-synuclein homeostasis; implications for Parkinson’s disease
OGA inhibition by thiamet G (TG) increases and α-synuclein accumulation, this can be partially reversed by MTOR inhibition by rapamycin. DIV7 primary rat cortical neurons were exposed to rapamycin (1 μM) and/or TG (0.25 μM) for 7 d. Western blot analyses of α-synuclein were performed. Ponceau staining was used as a loading control. Inhibition of OGA increased, and inhibition of MTOR decreased, α-synuclein levels. α-synuclein monomer is increased by TG, and decreased in TG + rapamycin compared to TG. Quantification of western blot band intensities was performed using NIH image J. *p < 0.05 compared to no TG, #p < 0.05 compared to –rapamycin, n = 3. Results were analyzed by ANOVA followed by Bonferroni’s Multiple Comparison post-hoc test