From: Neuroscience in the third dimension: shedding new light on the brain with tissue clearing
Mouse Stages | Immuno-staining | Clearing | Endogenous Fluorescence | Tissue shrinkage | Clearing Performance | Cost | Toxicity | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
<E15 | E15-P0 | Adult | Before Clearing | After Clearing | Clearing Solutions | Imaging Medium | ||||||
Solvents | ||||||||||||
3DISCO | Entire embryo | Isolated organs | Isolated organs | 1–3 wks | − | 1 d | − | +++ (up to 50%) | +++ | + | +++ | + |
iDISCO+ | Entire embryo | Entire embryo | Isolated organs | 2 wks | − | 2 d | − | + (up to 10%) | +++ | + | +++ | + |
uDISCO | − | − | Entire animal | 2 wks | − | 3 d - 1 wk | Yes (up to 4 wks) | +++ (up to 40%) | +++ | + | +++ | +++ |
Reagents | ||||||||||||
CLARITY | Entire embryo | Entire embryo | Isolated organs | − | 1–3 wks | 5 d - 2 m | Yes | * | ++ | +++ | +++ | − |
PACT | − | − | 3 mm slice | − | 1–2 wks | 2 wks | Yes (up to 2 m) | * | ++ | ++ | +++ | − |
PARS | − | − | Entire animal | − | 10 d | 4 d - 2 wks | Yes (up to 3 m) | * | ++ | ++ | +++ | − |