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Table 1 A hands-on comparison of tissue clearing protocols

From: Neuroscience in the third dimension: shedding new light on the brain with tissue clearing

  Mouse Stages Immuno-staining Clearing Endogenous Fluorescence Tissue shrinkage Clearing Performance Cost Toxicity
  <E15 E15-P0 Adult Before Clearing After Clearing       Clearing Solutions Imaging Medium
Solvents
 3DISCO Entire embryo Isolated organs Isolated organs 1–3 wks 1 d +++ (up to 50%) +++ + +++ +
 iDISCO+ Entire embryo Entire embryo Isolated organs 2 wks 2 d + (up to 10%) +++ + +++ +
 uDISCO Entire animal 2 wks 3 d - 1 wk Yes (up to 4 wks) +++ (up to 40%) +++ + +++ +++
Reagents
 CLARITY Entire embryo Entire embryo Isolated organs 1–3 wks 5 d - 2 m Yes * ++ +++ +++
 PACT 3 mm slice 1–2 wks 2 wks Yes (up to 2 m) * ++ ++ +++
 PARS Entire animal 10 d 4 d - 2 wks Yes (up to 3 m) * ++ ++ +++
  1. A comparison of most used clearing protocols. – = no information/ not applicable; + = short/low; +++ = long/high; * = varies between mounting media