Fig. 3
From: A T-type channel-calmodulin complex triggers αCaMKII activation
The Cav3-CaM association is lost upon Cav3 channel-mediated calcium influx. a, b Plots of FRET over time in tsA-201 cells coexpressing GFP-Cav3.1, mKate-CaM, and Kir2.1. Cells were exposed to Low [K]o (1 mM) or High [K]o (50 mM). a The FRET signal is stable in low [K]o for 11 min (upper plot) but is lost within 50 s upon perfusion of high [K]o (lower plot). b The decrease in FRET signal in high [K]o is blocked by expressing a Cav3.1 pore mutant that does not conduct calcium (upper plot), or 20 μM TTA-P2 (lower plot). c Bar plots of mean measures of FRET in the indicated conditions derived from 3 to 5 plates with 8–25 ROIs at time point 140 s. Mibefradil (Mib) 1 μM, Ni2+ 300 μM, BAPTA-AM 100 μM. Average values are mean ± SEM. * p < 0.05, ** p < 0.01, ns, not significant