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Fig. 4 | Molecular Brain

Fig. 4

From: The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress

Fig. 4

GPR55 knockdown abolished O-1602-mediated anxiolytic effects. a Immunohistochemistry image showing the GPR55 shRNA infected cells (GFP positive) in the MO cortex (Scale bar, 500 um; insert: scale bar, 100 um). b Western blot image showing the expression of GPR55 in GPR55 knockdown (GPR55 shRNA-injected group) in the MO cortex as compared to negative shRNA-injected group. **p < 0.01 versus control group; c In the EPM, O-1602 with negtive shRNA increased the duration and frequency in the open arms. O-1602 with GPR55 shRNA abolished the increase on duration and frequency in the open arms. **p < 0.01 versus control group; # p < 0.05 versus vehicle + negative shRNA group; & p < 0.05, && p < 0.01 versus O-1602 + negative shRNA group. d In the OFT, O-1602 with negtive shRNA increased the time in the center area and the total distance traveled. O-1602 with GPR55 shRNA reversed the effect on time in the center area and the total distance traveled. *p < 0.05, **p < 0.01 versus control group; # p < 0.05 versus vehicle + negative shRNA group; & p < 0.05versus O-1602 + negative shRNA group. e O-1602 with negtive shRNA decreased the expression of GluA1 and GluN2A, but GluN2B did not decrease. O-1602 with GPR55 shRNA abolished the decrease in GluA1 and GluN2A expression, although knockdown did not affect the expression of GluN2B. *p < 0.05, **p < 0.01 versus control group; ## p < 0.01 versus vehicle group; && p < 0.01 versus O-1602 + negative shRNA group. Each group contains 6–8 mice. Data are from three independent experiments

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