Fig. 1
mGlu2 Receptor protein levels in the cerebral cortex of mGlu2+/+ mice subjected to transient MCAO. Representative images of Nissl staining in coronal sections of mice at 3, 6, 12 and 48 h after monofilament-induced transient MCAO are shown in (a). The boundaries of dissection of the core region and the neighboring cortical region used for Western blot analysis are shown in (b). Immunoblot analysis of mGlu2 receptors in the cortical region corresponding to the core and in the cortical regions neighboring the core (and the corresponding regions of the contralateral hemispheres and of non-ischemic mice) is shown in (c) and (d), respectively. Densitometric data were obtained as the sum of the two bands corresponding to monomers and dimers. Data are means + S.E.M. of 4–5 mice per group. *p < 0.05 as compared to all other groups (One-way ANOVA + Fisher’s PLSD; F2,10 = 9.26)