Fig. 7
Improved motor asymmetry in Grm2 mutant Han Wistar rats subjected to transient MCAO. Asymmetry (expressed by laterality index, normalized to pre-ischemia values) in the PHR test is shown in (a). Kruskal-Wallis nonparametric ANOVA and Mann-Whitney comparisons between Et-1-infused and sham-operated rats: (*) for WT rats and (#) Grm2 mutant rats, respectively; Asymmetry in the preference and latency of removing the adhesive tapes in the AT test is shown in (b) and (c), respectively. Three-way GLM rANOVA (first factor ISCHEMIA with two levels: Et-1 and sham-operated, second factor GROUP with two levels: WT and Grm2 mutant rats, and third factor TIME with three levels: 1, 24 and 72 h) revealed a significant effect of factor ISCHEMIA for all rats, as follows: Preference (F(1,15) = 12.84, p = 0.003) and Latency (F(1,15) = 28.31, p = 0.00009). For ischemic rats (infused with Et-1), two-way rANOVA with factors GROUP (WT and Grm2 mutant rats) and TIME (1, 24 and 72 h) showed significant effect of the factor GROUP for both AT parameters, as follows: Preference (F(1,10) = 10.84, p = 0.008) and Latency (F(1,10) = 10.83, p = 0.008). * Fisher LSD comparisons between Et-1 and sham-operated WT rats and + between WT- Et-1 and Grm2 mutant-Et-1 rats. Number of rats: WT-sham-operated (n = 3); Grm2 mutant Han Wistar rats-sham-operated (n = 4); WT-Et-1 (n = 7); Grm2 mutant Han Wistar rats-Et-1 (n = 8). Significance was set at p < 0.05