Fig. 2

Characterisation of Moesin knockdown and overexpression. a–k. Immunohistochemistry with anti-Moesin (magenta) and anti-Myc (green) antibodies on whole mount brains. In all brains, elav-GAL4-mediated expression was restricted to the adult brain with the TARGET system. a–c. frontal confocal projection through a brain expressing Myc-Moe. d–f. Posterior confocal projection through a brain expressing Myc-Moe. g–i. Posterior confocal projection through a brain expressing Myc-MoeT559D. j, k. Confocal projection through a mushroom body expressing Myc-Moe (j) and Myc-Moe T559D (k). l. Western blot shows the expression of Moesin in head lysates of flies in which Moesin is overexpression or knocked down. The wild-type strain w1118 was also crossed to elav-GAL4 as a control genotype. Blots were probed with anti-Moesin to detect endogenous Moesin as well as Myc-Moe and Myc-MoeT559D. Anti-α-tubulin antibody was used as a loading control