Fig. 3

Altered Moesin expression disrupts mushroom body development. a–h. Immunohistochemistry with anti-FasII antibody on whole mount brains reveals mushroom body defects resulting from elav-GAL4 driven expression of UAS-Moe constructs. All images are frontal confocal projections through the mushroom body region of the brain. Scale bar = 50 μm. a. Wild-type mushroom body. α, β and γ lobes of the mushroom body are labeled in white. b. Misoriented β lobes (arrowheads) in a fly expressing Myc-Moe. c. Thin, reduced, α lobe projections in a fly expressing Moe-KD2. d. Complete disruption of mushroom body development in a fly expressing Myc-MoeT559D, with thin, distorted γ lobes (arrow). e. Missing α and β lobes (dashed lines) in a fly expressing Moe-KD1. f. Missing β lobe (dashed line) and α/β branching defect (arrow) in a fly expressing Moe-KD1. g. Axon stalling defect characterized by a partially formed α lobe (arrowhead) in a fly expressing Moe-KD2. h. β lobe outgrowth defect (arrow) in a fly expressing Moe-KD2. i. Misdirected α lobe (arrow) in a fly co-expressing Myc-Moe and Moe-KD2. j. Rescue of mushroom body development through coexpression of Myc-Moe with Moe-KD1. k. Thin α lobe (arrowhead) in a fly co-expressing Myc-Moe and Moe-KD1. l. Rescue of mushroom body development through coexpression of Myc-Moe with Moe-KD2