Fig. 1
From: The DLGAP family: neuronal expression, function and role in brain disorders
a Pictogram of chromosome 18, 8, 1 and 20 and the four DLGAP1–4 loci, respectively. DLGAP1 is located on chromosome 18 and transcript variants 1 to 9 are illustrated. DLGAP2 is found on chromosome 8 and has two transcript variants. The DLGAP3 gene is located on chromosome 1 and has one transcript variant. DLGAP4 is located on chromosome 20 and has 3 transcript variants. b Heatmap showing the homology in percentage between the longest isoform, isoform a, of DLGAP1–4 proteins respectively. The color key indicates the respective percentage to each colour. The heatmap is created in RStudio v.0.99.484 with data from a multiple alignment made with clustal omega v.1.2.1. DLGAP1 isoform a: 977 amino acids (NP_004737.2), DLGAP2 isoform a: 975 amino acids (NP_004736.2), DLGAP3 isoform a: 979 amino acids (NP_001073887.1) and DLGAP4 isoform a: 989 amino acids (NP_055717.2). c Phylogenetic tree of DLGAP1–4 protein conservation in following species; Homo sapiens (NP_004737.2, NP_004736.2, NP_001073887.1, NP_001035951.1), Macaca mulatta (AFE64413.1, AFJ72104.1, AFE64177.1), Bos taurus (NP_001179558.1, DAA17363.1, NP_001179367.1, AAI26739.1), Rattus norvegicus (NP_075235.3, NP_446353.2, NP_775161.2), Mus musculus (NP_808307.2, NP_766498.2, AAH57615.1, NP_001035953.1), Pongo abelii (XP_009251088.1, NP_001127321.1), Xenopus tropicalis (NP_001123829.1, NP_001106458.1, XP_012827022.1) and Danio rerio (NP_001189384.1, XP_009291347.1, NP_001038179.1, AAI33919.1). EV: Experimentally validated, CT: Conceptual Translation, P: Predicted from genomic sequence. The phylogenetic tree is created in RStudio v.0.99.484 with data from a multiple alignment made with clustal omega v.1.2.1. d Figure of multiple alignment of DLGAP1–4 GH1 domain and C-terminal. The sequence homology of the GH1 domain is 61 to 75% between DLGAP1 – DLGAP4. The alignment and data is generated in clustal omega v.1.2.1 and visualized in CLC sequence viewer v.7.6