Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis

Table 1 The composition of CS disaccharides in T1KO compared with WT

mol%	Average	SEM	Average	SEM	P value
Cerebrum	WT		KO
O	9.1	0.19	12.0	1.04	0.046
C	1.8	0.27	5.3	0.12	0.821
A	87.2	0.14	77.7	0.69	0.027
D	0.7	0.05	2.1	0.03	0.003
E	1.2	0.05	2.9	0.44	0.001
Total	100.0		100.0
Diencephalon	WT		KO
O	4.4	0.17	4.7	0.25	0.374
C	3.8	0.09	3.0	0.06	0.002
A	88.9	0.34	89.4	0.34	0.367
D	1.3	0.13	1.0	0.03	0.075
E	1.6	0.05	1.9	0.06	0.030
Total	100.0		100.0
Cerebellum	WT		KO
O	7.6	1.88	4.5	0.24	0.183
C	3.7	0.17	4.9	0.43	0.062
A	85.5	1.52	87.3	0.46	0.333
D	1.8	0.20	1.6	0.12	0.395
E	1.4	0.01	1.7	0.15	0.082
Total	100.0		100.0
Spinal Cord	WT		KO
O	12.0	1.04	11.8	2.68	0.930
C	5.3	0.12	4.7	0.06	0.041
A	77.7	0.69	78.7	2.27	0.647
D	2.1	0.03	1.4	0.25	0.036
E	2.9	0.44	3.4	0.59	0.525
Total	100.0		100.0
Visual Cortex	WT		KO
O	9.0	0.18	10.7	0.16	4E-04
C	2.7	0.01	2.3	0.03	3E-05
A	86.4	0.18	84.6	0.19	5E-04
D	0.7	0.03	0.4	0.03	4E-04
E	1.2	0.01	1.9	0.03	5E-07
Total	100.0		100.0

Note that among the five patterns of CS-derived disaccharides, CS-E in T1KO was significantly increased in each brain region. Statistically significant differences in T1KO are shown in italics (p < 0.05), compared to the corresponding results for WT [7, 8, 10]

ISSN: 1756-6606