Skip to main content

Table 2 The behavioral tests in T1KO that were not significantly different between genotypes (p ≥ 0.05)

From: Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis

Test

Controls

Mutants

F value

p value

Mean

SEM

Mean

SEM

General health/neurological screen

 body temperature (°C)

36.85

0.141

37.28

0.164

2.747

0.1049

 grip strength (n)

0.554

0.02

0.577

0.012

1.038

0.3141

 wire hang (latency to fall, s)

39.512

5.428

29.507

2.879

3.204

0.0808

Light/dark transition

 latency to light (s)

44.143

5.829

56.1

6.621

1.293

0.2619

 light distance (cm)

622.129

46.667

609.563

41.119

0.034

0.8548

 dark distance (cm)

1555.886

65.493

1628.983

35.355

1.147

0.2904

 number of transitions

32.714

2.23

28.967

1.965

1.32

0.2571

 stay time in light (s)

158.071

9.869

147.5

10.065

0.423

0.5189

Open field

 stereotypic counts

6911.643

520.353

7369.367

526.983

0.289

0.5939

 vertical activity

348.357

53.683

275.4

43.994

0.966

0.3312

 center time (s)

633.986

121.934

813.077

111.923

0.945

0.3365

Elevated plus maze

 number of total entry (times)

36.071

2.319

36.833

2.229

0.044

0.835

 entry into open arms (%)

13.393

2.567

13.863

1.572

0.027

0.8715

 total distance travelled (cm)

1806.893

92.545

1880.087

73.559

0.343

0.5614

 time on open arms (times)

3.443

1.125

2.923

0.744

0.152

0.6985

Hot plate

 latency (s)

6.403

0.386

6.318

0.394

0.018

0.8944

Social interaction (novel environment)

 duration of contacts (s)

68.7

9.138

88.707

7.29

2.605

0.1222

 number or contacts (times)

40.143

3.845

45.333

2.462

1.358

0.2577

 mean duration of contacts (s)

1.786

0.263

1.993

0.187

0.4

0.5341

 distance travelled (cm)

2957.586

285.871

3196.793

153.133

0.654

0.4283

Porsolt forced swim

 day1 immobility (%)

41.8131

3.617

46.252

2.314

1.099

0.3009

 day2 immobility (%)

57.747

3.627

54.268

2.624

0.569

0.4551

Gait analysis

 front swing/stride duration

41

0.508

40.969

0.43

0.002

0.9662

 front brake/stride duration

28.115

0.909

27.234

0.715

0.512

0.4784

 front propel/stride duration

30.896

0.871

31.795

0.645

0.635

0.4302

 front stance width

1.946

0.062

1.921

0.038

0.134

0.7164

 front step angle

62.308

1.762

60.066

1.075

1.269

0.2666

 front paw angle

−0.235

0.866

0.89

0.455

1.591

0.2144

 hind brake/stride duration

14.142

0.831

15.564

0.364

3.373

0.0737

 hind step angle

53.746

1.757

55.321

1.627

0.338

0.564

 hind paw angle

−0.788

0.953

−0.983

0.519

0.038

0.8471

Barnes maze

 time spent around target (probe test, 1 day)

40.538

4.748

39.143

4.067

0.042

0.8385

 time spent around target (probe test, 1 month)

32.154

5.78

30.172

4.224

0.072

0.7904

 time spent around target (reversal test, 1 day)

31.077

4.118

28.214

3.227

0.27

0.6064

Cued and contextual fear conditioning

 conditioning (freezing, %)

57.756

2.66

56.821

1.83

0.82

0.7764

 context test, 1 day (freezing, %)

70.082

3.444

66.333

3.372

0.453

0.5053

 cued test, with tone,1 day (freezing, %)

89.158

3.641

88.147

2.004

0.069

0.7945

 context test, 30 days (freezing, %)

79.412

5.225

79.481

3.222

0.0001376

0.9907

 cued test, pretone period, 30 days (freezing, %)

60.603

4.475

49.672

4.306

2.345

0.1342

 cued test, with tone, 30 days (freezing, %)

90.183

3.079

87.86

1.944

0.425

0.5186

Tail suspension

 immobility (%)

37.255

3.747

41.75

2.603

0.957

0.334

24 h home cage monitoring

 activity light period (A.U.)

478,882.065

30,477.038

670,800.611

107,540.908

2.948

0.1167

 activity dark period (A.U.)

1,033,998.833

66,355.759

1,385,925

191,066.128

3.027

0.1125

 number of particle dark period

1.175

0.02

1.196

0.022

0.482

0.5034

 

Stranger

Empty or Familiar

t value

p value (paired-t)

Mean

SEM

Mean

SEM

  

Social approach test (Stranger vs Empty)

 number of entry around cage (controls)

9

1.043

7.714

0.952

1.178

0.26

 time spent around cage (mutants)

209.241

12.352

137.448

10.928

0.627

0.5359

Social novelty preference test (Stranger vs Familiar)

 number of entry around cage (controls)

8.357

0.887

8.786

1.11

−0.405

0.6918

 number of entry around cage (mutants)

9.862

0.851

10.034

0.863

−0.16

0.8742

 time spent around cage (controls)

165.071

16.433

148.5

28.882

0.435

0.6708

 time spent around cage (mutants)

175.31

17.16

156.379

13.814

0.673

0.5063

  1. The tests that were significantly different between T1KO and WT are displayed in Fig. 4. The results of the tests that were not significantly different are listed here. Each group was composed of 20 male mice at the age of 12 weeks
Back to article page

Molecular Brain

ISSN: 1756-6606