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Table 2 The behavioral tests in T1KO that were not significantly different between genotypes (p ≥ 0.05)

From: Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis

Test Controls Mutants F value p value
Mean SEM Mean SEM
General health/neurological screen
 body temperature (°C) 36.85 0.141 37.28 0.164 2.747 0.1049
 grip strength (n) 0.554 0.02 0.577 0.012 1.038 0.3141
 wire hang (latency to fall, s) 39.512 5.428 29.507 2.879 3.204 0.0808
Light/dark transition
 latency to light (s) 44.143 5.829 56.1 6.621 1.293 0.2619
 light distance (cm) 622.129 46.667 609.563 41.119 0.034 0.8548
 dark distance (cm) 1555.886 65.493 1628.983 35.355 1.147 0.2904
 number of transitions 32.714 2.23 28.967 1.965 1.32 0.2571
 stay time in light (s) 158.071 9.869 147.5 10.065 0.423 0.5189
Open field
 stereotypic counts 6911.643 520.353 7369.367 526.983 0.289 0.5939
 vertical activity 348.357 53.683 275.4 43.994 0.966 0.3312
 center time (s) 633.986 121.934 813.077 111.923 0.945 0.3365
Elevated plus maze
 number of total entry (times) 36.071 2.319 36.833 2.229 0.044 0.835
 entry into open arms (%) 13.393 2.567 13.863 1.572 0.027 0.8715
 total distance travelled (cm) 1806.893 92.545 1880.087 73.559 0.343 0.5614
 time on open arms (times) 3.443 1.125 2.923 0.744 0.152 0.6985
Hot plate
 latency (s) 6.403 0.386 6.318 0.394 0.018 0.8944
Social interaction (novel environment)
 duration of contacts (s) 68.7 9.138 88.707 7.29 2.605 0.1222
 number or contacts (times) 40.143 3.845 45.333 2.462 1.358 0.2577
 mean duration of contacts (s) 1.786 0.263 1.993 0.187 0.4 0.5341
 distance travelled (cm) 2957.586 285.871 3196.793 153.133 0.654 0.4283
Porsolt forced swim
 day1 immobility (%) 41.8131 3.617 46.252 2.314 1.099 0.3009
 day2 immobility (%) 57.747 3.627 54.268 2.624 0.569 0.4551
Gait analysis
 front swing/stride duration 41 0.508 40.969 0.43 0.002 0.9662
 front brake/stride duration 28.115 0.909 27.234 0.715 0.512 0.4784
 front propel/stride duration 30.896 0.871 31.795 0.645 0.635 0.4302
 front stance width 1.946 0.062 1.921 0.038 0.134 0.7164
 front step angle 62.308 1.762 60.066 1.075 1.269 0.2666
 front paw angle −0.235 0.866 0.89 0.455 1.591 0.2144
 hind brake/stride duration 14.142 0.831 15.564 0.364 3.373 0.0737
 hind step angle 53.746 1.757 55.321 1.627 0.338 0.564
 hind paw angle −0.788 0.953 −0.983 0.519 0.038 0.8471
Barnes maze
 time spent around target (probe test, 1 day) 40.538 4.748 39.143 4.067 0.042 0.8385
 time spent around target (probe test, 1 month) 32.154 5.78 30.172 4.224 0.072 0.7904
 time spent around target (reversal test, 1 day) 31.077 4.118 28.214 3.227 0.27 0.6064
Cued and contextual fear conditioning
 conditioning (freezing, %) 57.756 2.66 56.821 1.83 0.82 0.7764
 context test, 1 day (freezing, %) 70.082 3.444 66.333 3.372 0.453 0.5053
 cued test, with tone,1 day (freezing, %) 89.158 3.641 88.147 2.004 0.069 0.7945
 context test, 30 days (freezing, %) 79.412 5.225 79.481 3.222 0.0001376 0.9907
 cued test, pretone period, 30 days (freezing, %) 60.603 4.475 49.672 4.306 2.345 0.1342
 cued test, with tone, 30 days (freezing, %) 90.183 3.079 87.86 1.944 0.425 0.5186
Tail suspension
 immobility (%) 37.255 3.747 41.75 2.603 0.957 0.334
24 h home cage monitoring
 activity light period (A.U.) 478,882.065 30,477.038 670,800.611 107,540.908 2.948 0.1167
 activity dark period (A.U.) 1,033,998.833 66,355.759 1,385,925 191,066.128 3.027 0.1125
 number of particle dark period 1.175 0.02 1.196 0.022 0.482 0.5034
  Stranger Empty or Familiar t value p value (paired-t)
Mean SEM Mean SEM   
Social approach test (Stranger vs Empty)
 number of entry around cage (controls) 9 1.043 7.714 0.952 1.178 0.26
 time spent around cage (mutants) 209.241 12.352 137.448 10.928 0.627 0.5359
Social novelty preference test (Stranger vs Familiar)
 number of entry around cage (controls) 8.357 0.887 8.786 1.11 −0.405 0.6918
 number of entry around cage (mutants) 9.862 0.851 10.034 0.863 −0.16 0.8742
 time spent around cage (controls) 165.071 16.433 148.5 28.882 0.435 0.6708
 time spent around cage (mutants) 175.31 17.16 156.379 13.814 0.673 0.5063
  1. The tests that were significantly different between T1KO and WT are displayed in Fig. 4. The results of the tests that were not significantly different are listed here. Each group was composed of 20 male mice at the age of 12 weeks
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