Fig. 3

Diversity of response properties across different morphological categories during cLTP. (a) Scatter plot (left) and representative example images (right) of mushroom type spines (n = 432) comparing raw value of head diameter measured before vs 45 min after treatment with 400 μM glycine. Black symbols = > 25% increase, white symbols = >25% decrease, gray symbols = changes outside this range. Bar graph inset represents distribution of these groups as percent of total (where black = increase, white = decrease, gray = no change). (b) Timecourse of changes in head diameter for mushroom spines represented as percentage of baseline values after glycine treatment alone (n = 432) or in combination with anisomycin (40 μM, n = 119) or rapamycin (200 nM, n = 129), compared to HBS treated controls (n = 261). (c) Mean (+SEM) head width of mushroom spines assessed 45 min after glycine-induced potentiation with or without pretreatment from anisomycin or rapamycin. Here, all values were normalized to the average spine head width of HBS treated controls at the 45 min time point only. *p < 0.05 relative to HBS treated controls. Remaining panels as indicated above, for stubby spines (d-f; control stubby n = 487, glycine stubby n = 467, glycine + aniso stubby n = 152, glycine + rap stubby n = 272), flat spines (g-i; control flat n = 169, glycine flat n = 184, glycine + aniso flat n = 92, glycine + rap flat n = 85), thin spines (j-l; control thin n = 273, glycine thin n = 301, glycine + aniso thin n = 92, glycine + rap thin n = 129), and filopodia (m-o; control fil n = 125, glycine fil n = 152, glycine + aniso fil n = 34, glycine + rap n = 64). Scale bar = 1 μm