Fig. 1

Whole cell patch-clamp recordings of layer II/III pyramidal neurons in the ACC of tree shrew. a Preparing process of tree shrew brain slices. Tree shrew was anesthetized with 1–2% isoflurane (upper); slices including ACC area from Bregma +3.30 to −2.03 mm (middle); representative coronal brain slice of tree shrew including ACC area (bottom). b and (c) Schematic diagram and representative recording diagram showing the placement of stimulating and recording electrodes in the ACC of tree shrew. d Representative photomicrograph of a biocytin-labeled pyramidal neuron in the layer II/III of ACC, scale bar: 50 μm