Fig. 2

Glutamatergic neuron mediated EPSCs in the tree shrew. a Identification of pyramidal neuron (upper) and interneuron (bottom) by injection of step currents (−50, 0, and 50 pA). b Monosynaptic EPSCs induced by 5 shocks at 5 Hz (upper) and 20 shocks at 20 Hz (bottom). c and (d) Sample traces and pooled data showed the input-output relationship of basal EPSCs in the ACC of tree shrew (n = 7 neurons/3 tree shrews). e EPSCs were recorded in the presence of picrotoxin (100 μM). After the perfusion of CNQX (20 μM) 10 min, a small residual current remained that could be totally blocked by CNQX and AP5 (50 μM) together. Sample traces (left) and sample time course points (right) showed the EPSCs in the presence of CNQX and AP5. d Statistical results showed that the percentage of EPSCs in the presence of CNQX and AP5 (n = 8 neurons/4 tree shrews). ***P < 0.001, error bars indicated SEM