Fig. 4
From: Criteria for identifying the molecular basis of the engram (CaMKII, PKMzeta)
Saturation test. a Path of rats (grey; superposition of six experiments) on circular platform before and after injection of control virus expressing GFP; shock zone (red pie shape) and individual shocks given (small red circles). Trial 1 is first training trial on day 1; trial 12 is last training trial (on day 4); reduction in number of shocks indicates learning. Trial 13 tests memory retention on day 10, 3 days after viral injection. Trials 14–16 show a deficit in relearning on day 10. b Path of rats before and after injection of virus expressing activated CaMKII (T286D/T305A/T306A). c Summary data. After viral expression of T286D/T305A/T306A, memory was poor (p = 0.02, D = 0.75) (n = 6) compared to GFP controls. After viral expression of GFP, memory was strong in one group measured at day 10 (n = 2) and in another group measured at day 16 (n = 6) (the groups are not significantly different, so data is combined here as “day 10” (n = 8)). For CaMKII*-injected animals, memory on day 10 was not significantly different than memory on day 16 for K42 M-injected animals (p = 0.8096, D = 0.3333). A two-sample K-S test was used to determine statistical significance. Error bars represent mean ± SEM. Asterisks indicate statistical significance (p < 0.05; Kolmogorov–Smirnov test). Reprinted with permission from [48]