Fig. 1

Loss of presynaptic gef26 function leads to synaptic overgrowth at the NMJ. a Confocal images of anti-HRP-labeled NMJs 6/7 (left) and NMJs 4 (right) in wild-type (w ¹¹¹⁸) and gef26 ⁶/Df third-instar larvae. gef26 mutant NMJs have increased numbers of total and satellite boutons compared with wild type. Arrowheads mark satellite boutons. Scale bars, 20 μm. b Quantification of total bouton number normalized to muscle surface area and satellite bouton number at NMJ 6/7 and NMJ 4 in wild-type, gef26 ⁶/gef26 ⁶, gef26 ⁶/Df, C155-GAL4/+; gef26 ⁶/Df; UAS-gef26/+ (Gef26 rescue-pre), and gef26 ⁶/Df; BG57-GAL4/UAS-gef26 (Gef26 rescue-post) third-instar larvae. The number of NMJs analyzed is indicated in each bar. Data are expressed as mean ± SEM. All comparisons are made with wild-type (*P < 0.001)