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Fig. 2 | Molecular Brain

Fig. 2

From: Accelerated super-resolution imaging with FRET-PAINT

Fig. 2

Comparison of the imaging speeds of DNA-PAINT and FRET-PAINT. a DNA-PAINT images reconstructed at specified acquisition time. b FRET-PAINT images of the same area as in (a) reconstructed at specified acquisition time. (c) The accumulated number of localized spots as a function of time for DNA-PAINT images of (a) (black boxes), and FRET-PAINT images of (b) (red boxes). The data are fitted to linear functions (solid lines). The slope of FRET-PAINT is 29-fold larger than that of DNA-PAINT. (d) Comparison of the number of localized single-molecule spots per second of FRET-PAINT and DNA-PAINT. Nine different areas were sequentially imaged using FRET-PAINT and DNA-PAINT, and analysed to get the graph. The error bars represent standard deviation. (e) Comparison of spatial resolution of DNA-PAINT and FRET-PAINT as a function of imaging time. Seven different imaging areas were analysed to calculate resolution for each image. The error bars represent standard deviation. To obtain 50-nm spatial resolution (horizontal dashed line), 800-s imaging time was required to obtain the same spatial resolution using DNA-PAINT. On the other hand, 22-s imaging time was required for FRET-PAINT when we used 30 nM donor strand, revealing 36-fold increase of imaging speed. Scale bars: 2 um

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