Skip to main content
Fig. 3 | Molecular Brain

Fig. 3

From: Accelerated super-resolution imaging with FRET-PAINT

Fig. 3

Multiplexing capability of FRET-PAINT. a Multiplexed imaging that uses a donor and acceptor strand exchange scheme. FRET-PAINT images of microtubule (b), and mitochondria (c) obtained using the scheme (a). The both images were obtained at excitation of the same donor (Alexa488) by a blue laser. (d) An overlaid image of b and c. (e) Multiplexed imaging without buffer exchange. All donor and acceptor strands are simultaneously introduced into the imaging chamber, but microtubules and mitochondria were imaged sequentially by using different excitation lasers. FRET-PAINT images of microtubule (f) and mitochondria (g) obtained using the scheme (e). Microtubule images were obtained with green laser excitation whereas mitochondria images were obtained with blue laser excitation. (h) An overlaid image of f and g. All FRET-PAINT images were reconstructed from 500 frames recorded at a frame rate of 10 Hz. MT, microtubule; MC, mitochondria; DS, donor strand; AS, acceptor strand. Scale bars: 5 um

Back to article page