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Fig. 2 | Molecular Brain

Fig. 2

From: Genetic dissection of the neuro-glio-vascular machinery in the adult brain

Fig. 2

Preferential labeling of astrocytes via intravenous CAV2. a Experimental timeline of intravenous CAV2-Cre injection into RCL-tdT animals. b Coronal sections through cortex and dentate gyrus showing the distribution of tdT-expressing cells at 3 weeks after CAV2-Cre injection into RCL-tdT mice. The scale bar is 50 μm. c Co-localization between an anti-GFAP antibody and tdT three weeks after CAV2-Cre injection into RCL-tdT mice (top) The scale bar is 10 μm. Co-localization between tdT and a GFP reporter expressed by lentiviral-GFAP-GFP-infected cells (bottom left). Plot of the proportion of tdT cells that co-localized with GFAP in cortex and DG (bottom right). d Plot of the proportion of wrapping type tdT+ cells in the different laminae of the dentate gyrus. e Histogram of the distance distribution between tdT-expressing cells and contacted CD31+ cells in the dentate gyrus. f Representative images of the dentate gyrus displaying tdT and GFAP distribution in the different laminae (left). Plot of the density of tdT+ cells in the different layers of the dentate gyrus (right). One-way ANOVA F(2,39) = 11.57, P < 0.001. LSD test Mol vs GCL, P = 0.819; Mol vs Hilus, P < 0.001; GCL vs Hilus P < 0.001. g Plot of the relative distribution of astrocyte density in the three laminae of the dentate gyrus. One-way ANOVA F(2,18) = 2.71, P = 0.094. Data are plotted as mean +/− SEM. * indicates P < 0.05, ** indicates P < 0.01. Scale bars are 10 μm. n = 4 animals per experiment

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