Fig. 3
IC and HC astrocytes express GlyT1. a: Focal application of glycine (1 mM, 0.5 s; triangles) elicited an IGly (EH = −85 mV; a1-2). The competitive GlyT1 agonist sarcosine (2 mM) reduced IGly (max) (a3). b: Single-cell RT-PCR revealed transcripts for GlyT1 in all IC astrocytes and about half of the HC astrocytes. Transcripts for GlyT2 were not found. c: Focal application of glycine (1 mM, 10 s; triangle) induced a long lasting inward current (EH = −85 mV; c1-2, top). Responses to test pulse (see Additional file 2: Figure S2A) were used to calculate RM at t0, t1, t5, and t10 (c1-2, bottom). Upon glycine application, RM was not reduced in IC and HC astrocytes (c3). The number of recorded cells (n) is given within the diagrams. Shown are mean values ± SEM. Significance levels in c3 were Šidák corrected for three comparisons (see Methods section). bp: base pairs