Fig. 5
IC and HC astrocytes express GAT-1 and GAT-3. a: Focal application of GABA (1 mM, 0.5 s; triangles) elicited an IGABA (EH = −85 mV; a1-2). The non-competitive antagonists for GAT-1 (NO711, 10 μM) and GAT-3 (SNAP5114, 40 μM) reduced IGABA (max) (a3). b: Single-cell RT-PCR revealed transcripts for GAT-1 and GAT-3 in all IC astrocytes and about half of the HC astrocytes. Transcripts for GAT-2 and BGT-1 were never present. c: Focal application of GABA (1 mM, 10 s; triangle) induced a long lasting inward current (EH = −85 mV; c1-2, top). Responses to test pulse (see Additional file 2: Figure S2A) were used to calculate RM at t0, t1, t5, and t10 (c1-2, bottom). Upon GABA application, RM was transiently reduced exclusively in HC astrocytes (c3). The number of recorded cells (n) is given within the diagrams. Shown are mean values ± SEM. Significance levels in panel c3 were Šidák corrected for three comparisons (see Methods section). bp: base pairs