Fig. 2

Pro-inflammatory CKs modulation of synaptic activity in organotypic spinal cord cultures is prevented by MT2. In (a) and (b) Spontaneous PSCs (sPSCs) recorded from organotypic spinal cord ventral interneurons in control (CTRL) or after CKs treatments in the absence in (a) or in the presence in (b) of MT2. Note that CKs ability to increase sPSCs occurrence is prevented by MT2 applications. c The plot summarizes the frequency of sPSCs prior and after CKs incubation, note the significant increase in network activity, and the frequency of sPSCs prior and after CKs incubation in the presence of MT2, note the absence of significant changes in network activity. Small symbols depict data for six different culture series (between 5 and 8 cells each); larger symbols report mean values. In the inset: the frequency of sPSCs is expressed in respect to each control group, note the increase by 55.27% in CKs4H and by 65.94% in CKs6H. Virtually no increases were observed in MT2: 9.20% in CKs4H + MT2 and 8.63% in CKs6H + MT2. d Box-plots summarize the mEPSC event frequency and amplitude values in control or after CKs treatments. Note that CKs applications did not affect these parameters. See the Additional file 1: Figure S1 for mEPSCs in MT2 control and MT2 CKs-treated organotypic slices