Fig. 1
From: Foxg1 deletion impairs the development of the epithalamus
Foxg1 is required for the development of the epithalamus. (a and b): In situ hybridization of Foxg1 (a) and immunofluorescence of Foxg1 (b) in E12.5 coronal sections. Foxg1 is expressed at the dorsal developing diencephalic ventricular zone, particularly at the prospective habenular ventricular zone (arrows) and the pineal gland (arrowhead). (c): Foxg1 is effectively eliminated in Foxg1 mutants at E12.5. (d and e): In situ hybridization of Foxg1 (d) and immunofluorescence of Foxg1 (e) in E18.5 coronal sections. Arrow in d show the expression of Foxg1 in the habenula. (f): Foxg1 is effectively ablated at E18.5. (g-h’): In situ hybridization of E12.5 (g-g’) and E14.5 (h-h’) forebrain with Brn3a showing the habenula were slightly enlarged. (i, i’): Nissl staining of E14.5 forebrain in coronal sections revealed the enlarged habenula. The white dashed line in d, f, h and h’ outlined the diencephalon. (j-k’): Nissl staining in E18.5 coronal (j-j’) and sagittal sections (k-k’) showing the dorsolaterally expanded habenula (j, j’, arrows), enlarged third ventricle, more branched third ventricle choroid plexus (k, k’, asterisk) and the aberrant shape of the pineal gland in mutants. (l, l’): In situ hybridization revealed the Fzd10 + habenular region were enlarged. (m, m’): Immunofluorescence with GFP showing the expanded habenula in the mutants (m’) compared to that in the controls (m), the elongated habenular commissure (bracket) and the abnormal pineal gland. (n): Measurement of the volume of the habenula (n = 8, ** p = 0.0039). 3rdV, third ventricle; 3rdChp, third ventricle choroid plexus; 3rdVZ, ventricular zone of the third ventricle; Hb, habenula; Hbc, habenular commissure; MHb, medial habenula; Pg, pineal gland. Scale bars: 100 μm