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Fig. 2 | Molecular Brain

Fig. 2

From: Foxg1 deletion impairs the development of the epithalamus

Fig. 2

Abnormal development of the habenular subtype neurons. (a-a’): Immunofluorescence of anti-Pax6. Pax6+ progenitors in the epithalamic VZ (white arrowhead) were remarkably increased compared to those in the controls, and more Pax6+ cells were scattered in the dMHb (dotted line) and LHbL (yellow arrowhead). (b-b’): Increased number of CR+ subtype neurons in the dMHb (white dotted line) and LHbL (yellow dotted line). (c, c’): Increased numbers of CB+ subtype neurons in the habenula. (d, d’): In situ hybridization showing Brn3a strongly expressed in postmitotic neurons in the MHb (white dotted line) and weakly expressed in the LHb (black dotted line), and both stained areas were expanded with irregular morphologies in the Foxg1 mutants. (e-f’): Substance P-ergic neurons in the dMHb showing Tac1 in situ hybridization and Etv1+ glutamatergic neurons in the vMHb. (g-h’): Enlarged LHbMC and LHbL. (i-m): Quantitative analysis of the numbers of Pax6+ progenitors (i, n = 6, **p = 0.002), CR+ neurons (j, n = 6, Hb, **p = 0.0085; dMHb, *p = 0.0148; LHbL, **p = 0.0069), CB+ neurons (k, n = 6, ***p < 0.0001), and Tac1+ neurons (m, n = 8, p = 0.067). (l) Quantitative analysis of Brn3a+ area in coronal section crossing the midmost level of the habenula (l, n = 4, **p = 0.0063). The data are presented as the mean ± S.E.M. Hb, habenula; MHb, medial habenula; dMHb, dorsal medial habenula; vMHb, ventral medial habenula; LHb, lateral habenula; LHbL, lateral division of the lateral habenula; LHbMC, central part of the medial division of the lateral habenula. Scale bars: 100 μm

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