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Fig. 4 | Molecular Brain

Fig. 4

From: Foxg1 deletion impairs the development of the epithalamus

Fig. 4

Foxg1 is required for early epithalamic sub-regionalization. (a-b”): In situ hybridization of Dbx1 showing that the habenular progenitor region (bracket) was obviously expanded and shifted dorsolaterally after Foxg1 deletion. (a”’, b”’) Whole-mount in situ hybridization for Dbx1 at E12.5 The bracket indicates the epithalamus. (c-d”): Expanded Ngn2+ habenular VZ (black bracket) but normal pTH-C (red bracket), pTH-R (yellow bracket) and ZLI (arrow) in the mutants. (c”’, d”’): Whole-mount in situ hybridization for Ngn2 at E12.5. The bracket indicates the epithalamus. (e-f”): Smaller pineal recess (black bracket) shown by in situ staining of Fzd10. The red bracket marked the Fzd10weak strip between the pineal recess and the habenular ventricle zone. (g-g’): In situ hybridization of Mash1 showing normal pTH-R (arrow) and ZLI. (h-j’): No obvious changes in the patterning of p1 were revealed by the in situ staining with Pax3, which labels the pretectal VZ (h, h’); Lhx1, which labels the mantle zone of the caudal pretectum (i, i’); and BHLHB4, which labels the mantle zone of the rostral pretectum (j, j’). The black dashed line in h-j’ outlined the diencephalon. Epi, epithalamus; Hb, habenula; pTH-C, caudal progenitor domain of the thalamus; pTH-R, rostral progenitor domain of the thalamus; Pr, pineal recess; p1, prosomere 1; cPT, caudal pretectum; rPT, rostral pretectum; ZLI, zona limitans intrathalamica. Scale bars: 100 μm (scale bar in a”’, b”’, c”’ and d”’: 2 mm; g and g’: 200 μm)

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