Fig. 5
From: Foxg1 deletion impairs the development of the epithalamus
Shh and Fgf signalling were not affected in the epithalamic development. (a-a’): Transcription level of Fgf15 in the presumptive habenula (bracket) was not obviously affected in the mutants, no changes were observed in the region of pTH-R (arrowhead) in p2. (b-b’): The whole mount in situ from E12.5 embryos also showing a comparable transcription level in the epithalamus (bracket). c: Relative mRNA levels of Fgf15 (n = 4, p = 0.898). (d-e”): No obvious differences in Fgf8 were observed in the Foxg1 mutants. The white dashed line outlined the diencephalon. (f-h’): Staining of Wnt3a in dorsal P2 and Wnt8b in TE and P3 in mutants were comparable to that of controls. Arrows in f and f’ indicate the cortical hem, arrowheads in g and h indicate the thalamic eminence. (i-l”): The activity of the Shh signalling pathway appeared normal in the mutants. The white arrow in i and i’ indicates the ZLI. CH, cortical hem; Epi, epithalamus; Hb, habenula; IPN, interpeduncular nucleus; TE, thalamic eminence; ZLI, zona limitans intrathalamica. Scale bars: 100 μm (scale bar in b and b’: 1 mm, g-h’: 200 μm)