Fig. 5

RvD2 promoted ERK/NOS pathway in GPR18-dependent manner after MCAO/R. a–h The protein levels of pERK 1/2, ERK1/2, ZO-1, nNOS, and eNOS were assessed by Western blot and quantified. The mean values of the protein levels of pERK1/2, ERK, ZO-1, nNOS, and eNOS in the sham group were normalized to 1.0. Data are means ± SEM, n = 6. **P < 0.01 vs. sham group, ^#P < 0.05, ^##P < 0.01, ^&P < 0.05, ^&&P < 0.01. N.S. = no significant differences. i and j Double immunofluorescence analysis was performed with nNOS antibodies or eNOS antibodies (green) and neuron marker (NeuN, red) or endothelial cell marker (vWF, red), and the nuclei were fluorescently labeled with 4,6-diamino-2-phenylindole (DAPI) (blue). Arrows point to nNOS-positive neurons or eNOS-positive endothelial cells. Scale bar = 32 μm. The relative fluorescent intensity of nNOS in neurons and eNOS in endothelial cells was quantified (k and l). The mean value of the intensity of fluorescence in the sham group was normalized to 1.0. Data are means ± SEM, n = 6. *P < 0.05 vs. sham group, ^#P < 0.05, ^&P < 0.05. N.S. = no significant differences