Fig. 2
sAβ1–42 suppressed the intersynaptic vesicle movement and synapsin. a-c Neurons expressing GFP-synaptophysin were treated as indicated and imaged for 1 min to track the intersynaptic vesicles. a Representative kymographs showing trafficking of GFP-synaptophysin in each group. b MSD curves versus time. c Diffusion coefficients, n = 7 independent experiments for each group. d-g Neurons were transfected with GFP-synapsin (DIV8) and treated as indicated at DIV14. A single bouton was selectively photobleached and its recovery time was measured through FRAP assay. d Representative time-lapse images of FRAP assay for control, sAβ1–42 and sAβ1–42 with 6E10 (scale bar = 5 μm). White arrowheads indicate bleached boutons. e Average plots of fluorescence intensities (normalized to initial intensities) before and after photobleaching. f Fluorescence recovery traces in (e) were fitted to a single exponential function with time constant (τ) of 19.36±0.84 s for control, 28.92±1.8 s for sAβ1–42, and 21.38±1.82 s for sAβ1–42 with 6E10, respectively (n = 4 independent experiments for each group). g Mobile fractions were calculated by averaging final 5 values in (e). Mobile fraction (%): 68.10±2.67 for control, 55.13±5.47 for sAβ1–42, and 75.25±7.85 for sAβ1–42 with 6E10 (n = 4 independent experiments for each group). Values are means±SEM. N.S = no significant difference, * p < 0.05, ** p < 0.01 (ANOVA and Tukey’s HSD post hoc test))