Fig. 3
sAβ1–42 increases heterogeneity of presynaptic terminals. a-e Neurons were treated as indicated and immunostained for endogenous synaptophysin and bassoon. a Representative immunocytochemistry images (scale bar = 2 μm). b The number of presynaptic boutons were counted along the axon (Average bouton numbers per 100 μm axon length: 35.54±1.43 for control, 34.57±0.97 for sAβ1–42, and 37.92±1.53 for sAβ1–42 with 6E10 (n = 4 independent experiments for each group)). c The intensity of bassoon puncta (> 80,000) was measured and presented with the box plot. d The intensity profiles of synaptophysin-stained boutons were fitted into gamma distribution with a fixed shape parameter α = 4. The scale parameter β was as follows: Control = 29.56 (81,356 boutons); sAβ1–42 = 40.70 (89,599 boutons); sAβ1–42 with 6E10 = 28.20 (83,369 boutons). e Raw data of (d) were fitted to gamma function and their mean bouton intensity (α x β) and variance (α x β2) were measured. Values are means±SEM. N.S = no significant difference, ** p < 0.01 (ANOVA and Tukey’s HSD post hoc test)