Fig. 3

Expression of microglial in the spinal dorsal horn in WT and TLR4 KO mice. a The expression of microglia in the spinal dorsal horn was measured by immunohistochemistry (IHC) with Iba1 antibody. The number of microglia were significantly higher in WT Ipsi (A3, A4) superficial laminae of the dorsal horn than in the contralateral group (A1, A2). No significant increase in Iba1-immunoreactive (IR) cells was assessed in TLR4 KO mice. Scale bar = 50 μm in A1, A3, A5, and A7. Scale bar = 20 μm in A2, A4, A6, and A8. b The density of microglia in the superficial dorsal horn of mice was quantified with ImageJ. Two-way ANOVA; all the data are shown as mean ± SEM, where ***P < 0.001 denotes a significant difference compared with the control group